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hil 17af  (R&D Systems)


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    Structured Review

    R&D Systems hil 17af
    Hil 17af, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+human+il+17a+f/Recombinant+Human+IL-17A%2FF+Heterodimer+Protein/pmc12829784-62-7-8
    Average 94 stars, based on 5 article reviews
    hil 17af - by Bioz Stars, 2026-09
    94/100 stars

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    Cell Culture:

    Article Title: IL-17A binding agent and uses thereof
    Article Snippet: .. Materials and Equipment: Hs27 cells: ATCC Cat. No. CRL-1634 (Note: cells cultured for more than six weeks are not recommended for the bioassay); Hs27 cell culture medium: DMEM+10% FBS DMEM: ATCC Cat. No. 30-2002; FBS: GIBCO Cat. No. 10099, lot 8122818; Recombinant human IL-17A (rhIL-17A): R&D Systems Cat. No. 317-ILB, lot SOA161109B; Recombinant human IL-17A/F (rhIL-17A/F): R&D System Cat No. 5194-IL/CF, lot RXT101109A; Human CXCL1/GRO alpha Quantikine PharmPak kit: R&D system Cat. No. PDGR00 Equipment: Biotek ELx808 microplate reader. ..

    Bioassay:

    Article Title: IL-17A binding agent and uses thereof
    Article Snippet: .. Materials and Equipment: Hs27 cells: ATCC Cat. No. CRL-1634 (Note: cells cultured for more than six weeks are not recommended for the bioassay); Hs27 cell culture medium: DMEM+10% FBS DMEM: ATCC Cat. No. 30-2002; FBS: GIBCO Cat. No. 10099, lot 8122818; Recombinant human IL-17A (rhIL-17A): R&D Systems Cat. No. 317-ILB, lot SOA161109B; Recombinant human IL-17A/F (rhIL-17A/F): R&D System Cat No. 5194-IL/CF, lot RXT101109A; Human CXCL1/GRO alpha Quantikine PharmPak kit: R&D system Cat. No. PDGR00 Equipment: Biotek ELx808 microplate reader. ..

    Recombinant:

    Article Title: IL-17A binding agent and uses thereof
    Article Snippet: .. Materials and Equipment: Hs27 cells: ATCC Cat. No. CRL-1634 (Note: cells cultured for more than six weeks are not recommended for the bioassay); Hs27 cell culture medium: DMEM+10% FBS DMEM: ATCC Cat. No. 30-2002; FBS: GIBCO Cat. No. 10099, lot 8122818; Recombinant human IL-17A (rhIL-17A): R&D Systems Cat. No. 317-ILB, lot SOA161109B; Recombinant human IL-17A/F (rhIL-17A/F): R&D System Cat No. 5194-IL/CF, lot RXT101109A; Human CXCL1/GRO alpha Quantikine PharmPak kit: R&D system Cat. No. PDGR00 Equipment: Biotek ELx808 microplate reader. ..

    Article Title: Chronic mucocutaneous candidiasis and connective tissue disorder in humans with impaired JNK1-dependent responses to IL-17A/F and TGF-β.
    Article Snippet: .. After 24 hours, cells were left unstimulated or were stimulated with recombinant human IL-17A (317-ILB; R&D Systems), recombinant human IL-17F (1335-IL; R&D Systems), recombinant human IL-17A/F (5194-IL; R&D Systems), recombinant human TNF- (210-TA; R&D Systems), recombinant human IL-1 (201-LB; R&D Systems), recombinant human lymphotoxin 1/2 (8884-LY; R&D Systems), lipoteichoic acid from Staphylococcus aureus (LTA-SA) (tlrl-slta; InvivoGen), Pam3CSK4 (tlrl-pms; InvivoGen), fibroblast-stimulating lipopeptide-1 (FSL-1) (tlrl-fsl; InvivoGen), Pam2CSK4 (tlrl-pm2s-1; InvivoGen), and lipopolysaccharide (LPS) (L9764; Sigma-Aldrich) for a further 24 hours. .. Enzyme-linked immunosorbent assay (ELISA) kits were used to determine the levels of GRO- (DY275; R&D Systems), IL-6 (88-7066; Invitrogen), and IL-8 (M9318; Sanquin) in the supernatants.



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